FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_22.35100000083714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_22.35100000083714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12242618
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG447170.36525684293996596No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA242510.19808671641964162No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA172420.1408358898399019No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG165800.13542854967785484No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG164280.13418698516934857TruSeq Adapter, Index 22 (97% over 40bp)
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT156990.12823237644105207No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG141370.11547366747863896No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG140620.11486105341194179No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA137070.11196134682957518No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC135390.11058909132017351No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC134450.10982128168991305No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT124200.10144888944505169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA22750.033.6273335
CCGTACG23150.032.85016633
CGTACGT23100.032.63066534
GCACACG25600.031.11340511
ACGTCTG26550.029.8307515
TACGTAA25600.029.70787836
CACACGT28200.028.56336212
TCGTATG26500.028.27358245
CTCGTAT28600.026.59090844
CACGTCT30800.025.78744114
TACTCTA46800.025.6724454
TCTAGTT49250.025.4004927
ACGTAAT30450.025.12386937
AGCACAC32800.024.62662310
GTACTCT49400.024.0479933
ACCGTAC32550.023.91714732
TAGTTGT51400.023.7692589
TTTTTCC106950.022.8317741
GTTACCT53050.022.26681714
AGTCACC34700.022.24063328
Produced by FastQC (version 0.11.2)