FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_21.351000000836f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_21.351000000836f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9849128
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG510170.5179849424233293No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA263480.2675160684275806No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG189140.19203730523148851No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA182330.18512298753757692No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG149140.15142457281497407TruSeq Adapter, Index 21 (97% over 40bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG147980.15024680357489517No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT141500.14366754092341982No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG141100.14326141359925468No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA140120.14226640165505006No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC137130.1392305999069156No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC117650.1194521992200731No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG115150.11691390344404093No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT114060.11580720648569091No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC110720.11241604332891196No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG101360.10291266394344759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG24750.034.18139611
GTCACGT23650.033.67806229
AGTCACG24400.033.19614428
TCGTATG24300.032.68544845
ACGTCTG25600.032.60751715
CGTTTCG25450.031.38404733
CTCGTAT25800.031.30839244
CACGTCT27850.029.89221614
AGCACAC29000.029.4048110
GTACTCT44650.028.3704533
CACGTTT29100.027.67979231
CACACGT30600.027.64643912
TCTAGTT47200.027.4090637
TTTTTCC95350.026.815431
ACGTTTC30550.026.43953132
TAGTTGT48350.026.4316719
CAGTCAC31300.026.1656827
TACTCTA49900.025.5208594
ACACGTC33100.025.35441413
GAGCACA35400.024.4697919
Produced by FastQC (version 0.11.2)