FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_18.3510000008369c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_18.3510000008369c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1700265
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG50210.2953069080408054No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA28730.16897365998829594No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG20350.11968722522665584TruSeq Adapter, Index 18 (97% over 40bp)
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA19800.11645243535566516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC2150.039.76796335
CGTCCGC2550.034.4101933
CCGCACA2550.033.5298536
CGCACAT2650.032.26457237
GCACACG2950.031.27251411
GTCCGCA3100.028.3068234
CACACGT3600.025.62608712
ACGTCTG3550.025.35393515
AGTCACG3550.024.71717828
TTTTTCC13800.022.5055891
ACGTCCG4050.022.22120732
TACTCTA4100.021.9508614
GTCACGT4000.021.93649729
CTCGTAT3850.021.62366144
TCGTATG3900.021.34580645
ACACGTC4400.020.96679913
TCACGTC4400.020.4536130
CGGGCGC2800.019.287115
TCTAGTT5250.019.2848327
CACGTCT4800.019.22013314
Produced by FastQC (version 0.11.2)