FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_17.3510000008368f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_17.3510000008368f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3571087
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG167940.4702769772901081No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA77290.21643269962339198No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA53760.15054239787493276No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG50920.1425896372729088No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC49290.13802520073019783TruSeq Adapter, Index 3 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG45330.1269361401724461No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC41670.11668715995997857No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT39550.11075059218663673No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG37900.1061301502875735No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT37430.10481402441329488No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA37020.10366591460807312No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT36410.10195775123932854No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC36230.10145370303215799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7300.033.28757544
ACGTCTG8150.031.19666915
GTATGCC7800.031.1537645
TCGTATG7900.030.75940743
TCACGTA7800.030.57726530
GCACACG8550.029.47401611
AGTCACG8300.028.73526228
ACGTAGA8500.028.05913732
CACACGT9000.028.00031512
TACTCTA14450.027.2490584
CTCGTAT9200.026.65753242
CGTAGAG9200.025.67855633
ACACGTC9850.025.58404513
ACTCTAG18600.024.7983195
GTCACGT9800.024.79619629
GTACTCT15900.024.7640823
AGCACAC10900.024.3580710
TCTAGTT16450.024.2089827
CACGTCT10750.023.65142814
CACGTAG10150.023.49779931
Produced by FastQC (version 0.11.2)