FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_15.3510000008364c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_15.3510000008364c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3668028
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG140670.3835030703146214No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA76230.20782284104701493No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA54530.14866298730544042No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG53230.14511884860202812No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG45200.12322697645710448No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT43230.11785624319116429No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC43120.1175563545316448No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA41440.11297623682261967No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG41130.1121310960548829TruSeq Adapter, Index 15 (97% over 40bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG39970.10896863382722269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG7300.029.89646311
ACGTCTG7750.028.45124215
TCGTATG7650.026.17648745
AGCACAC8750.025.97073610
CACACGT8850.024.66035812
CTCGTAT8500.023.82354444
TTTTTCC32350.023.7259031
CACGTCT9500.022.97338514
GTACTCT14500.022.6551883
TAGTTGT14850.022.5751559
ACACGTC9800.022.49940113
CTCTAGT16750.021.760916
TTTTCCA36300.021.508572
GTCACAT9650.021.45108229
TCTAGTT15450.021.4071967
TACTCTA15450.021.1165184
ACTCTAG17450.020.8882665
TGTACTC16200.020.6947382
TTGTACT16450.020.2506281
GGCGGGC7050.020.10584613
Produced by FastQC (version 0.11.2)