FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_12.351000000835fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_12.351000000835fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872945
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG187220.6516657993800786No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA80290.2794693250305871No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG69010.24020647802168157No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT55690.19384290336222934No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA55660.19373848089678014No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG54120.188378127670387No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC50830.17692646395945624No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA50710.17650877409765936No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG49950.1738634049729459No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG44980.156564083196859No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC44450.15471928630725615No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT41040.1428499327345285No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG40740.14180570808003634No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC39240.13658458480757552No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC38990.1357143975954987No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG33710.11733604367643656No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC33070.11510836441351992TruSeq Adapter, Index 12 (100% over 51bp)
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA31460.1095043587677453No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT31250.10877340150960077No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG31000.10790321429752396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5150.031.02001444
ACGTCTG6400.028.82868215
TACTCTA16000.028.827684
GCACACG6500.028.38417611
GTACTCT16000.028.2651883
TCTAGTT17300.027.5708687
ACTCTAG17950.027.3254815
GTATGCC6000.027.0005245
CACACGT7100.026.3024112
TCGTATG6100.026.1890343
CTCTAGT18750.026.159146
TTTTTCC35700.025.0923791
CGGCAAT8350.025.068221
TTGTACT18650.024.4987661
TAGTTGT18750.024.4791959
TGTACTC18550.024.38012
ATCGGGC7200.024.3741996
GTTACCT18550.024.13793214
CACGTCT7650.023.8239914
TGTTACC19150.023.38083813
Produced by FastQC (version 0.11.2)