FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_11.351000000835d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_11.351000000835d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34085491
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG2592900.7607048993367882No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA1010400.29643111199425No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG922840.27074276266109826No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT760970.22325334847017458No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTT704830.20678299749298024No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA703740.20646321333613765No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA654430.19199664748851647No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG628970.184527193696579No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC609800.17890309985559547No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC592320.1737748181476981No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG573070.16812725390988206No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG541080.15874202897649325No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT527280.15469338552289008No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG516940.15165983673229175No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC515720.15130191318059638No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC488950.14344813164052705TruSeq Adapter, Index 11 (100% over 51bp)
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC474110.1390943730281016No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTAT439150.1288378096123069No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG409220.1200569473973545No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA389300.11421281858606644No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG371850.10909333827698124No Hit
CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGA358480.10517084820635267No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT345900.10148012830444485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC72300.033.42344
GCACACG77700.032.86709211
ACGTCTG82800.030.57079515
GTATGCC81300.029.83373845
AGTCACG81700.029.71534328
CACACGT86400.029.58325612
GTCACGG82150.029.5525729
TACTCTA185900.029.386434
CACGGCT82350.029.34418531
TTTTTCC475650.028.9769921
TCTAGTT194200.028.7444367
TCGTATG84700.028.66272543
GTACTCT190150.028.492973
CGGCAAT122900.028.3481481
ACTCTAG203900.027.675345
CACGTCT92250.027.39037114
CTCTAGT210100.027.1154296
CGGCTAC90900.026.53415733
TCGGGCG128250.026.2803237
CTCGTAT92350.026.26402742
Produced by FastQC (version 0.11.2)