FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_10.351000000835b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_10.351000000835b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4049174
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG175820.4342120145985329No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA99820.24651941358904308No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA67730.167268682452273No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC52600.1299030370144627TruSeq Adapter, Index 10 (100% over 51bp)
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG52500.12965607306576601No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC51820.12797671821462847No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG46140.11394916592865607No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44610.11017061751359661No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT43450.10730583570871491No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA42510.10498437459096595No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG40930.10108234420155814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG9100.029.17591311
CGTATGC8600.027.99461644
CACACGT9750.027.23051312
ACGTCTG9900.027.04620415
CACTAGC9100.026.95029831
TCGTATG9350.025.74905843
GTATGCC9200.025.67974545
CTCGTAT9650.025.18173442
TCTAGTT16200.024.999157
TCACTAG9800.024.56609730
TAGCTTA10200.023.82419234
TTTTTCC39950.023.3236271
CACGTCT11200.023.30421614
TACTCTA15750.023.1417834
ACACGTC11750.022.40404713
TAGTTGT16900.022.2332449
AGCACAC12400.021.77426110
AGTCACT11900.021.55442228
TATCTCG11150.021.39046339
GTACTCT17600.021.2206063
Produced by FastQC (version 0.11.2)