FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_09.35100000083580.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_09.35100000083580.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876968
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG117620.3033814052630819No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA75790.19548781418881972No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC67810.1749047193580138TruSeq Adapter, Index 9 (100% over 51bp)
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA52960.1366015917593336No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC41970.1082546980011184No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT40620.10477259549214749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7900.034.46189544
AGTCACG8550.032.368328
CTCGTAT8450.031.68627242
GTATGCC8750.031.1141745
ACGTCTG8800.030.93738615
GCACACG8900.030.84337811
GTCACGA8950.030.67027729
TCGTATG9450.028.80941843
CACGTCT10700.025.4438314
AGCACAC11050.024.84217510
CACACGT11150.024.82053612
TTTTTCC27300.024.402441
GATCTCG11450.023.77751239
ACACGTC11900.023.06713913
GAGCACA13050.021.5516439
TCTAGTT11150.020.986477
CGCGTAT3450.020.8754121
TTTTCCA33250.020.6392882
CAGTCAC13550.020.59006327
TCTCGTA13200.020.45420641
Produced by FastQC (version 0.11.2)