FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_08.35100000083566.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_08.35100000083566.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14495486
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG653280.4506782318302401No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT362330.24996057393315407No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG356370.2458489491142277No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA350110.24153036331448288No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG276120.19048688674529435No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA267140.18429185471946233No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG266520.1838641353591042No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC253770.1750682936743204No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC253320.17475785220309276TruSeq Adapter, Index 8 (100% over 51bp)
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA249010.1717845127786678No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC230850.1592564747397914No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC202660.13980904124221843No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG200850.13856037665794718No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG168720.116394855612292No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC167360.11545663249924838No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG165700.11431144840538633No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA160650.1108276052282759No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG158080.1090546394925979No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT156870.10821989686996351No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145180.10015531731740487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC36000.033.93739744
GCACACG38850.033.01159711
GTATGCC37600.032.732545
TCGTATG38100.032.24443
ACGTCTG41900.030.55490715
CTCGTAT41850.029.62356842
CACACGT45250.028.24281512
CACGTCT46400.027.97953814
TCTAGTT83900.027.9701377
AGCACAC46600.027.90773810
TACTCTA80300.027.879844
GTACTCT80600.027.8318963
TAGTTGT83300.027.2802689
CTCTAGT90150.026.95496
CGTGTGC36200.026.6022244
ACTCTAG92550.026.1348045
TGTACTC86050.026.04312
ACACGTC50100.025.8680113
TTGTACT90550.024.6822281
TTTTTCC136100.024.3098771
Produced by FastQC (version 0.11.2)