FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_06.35100000083530.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_06.35100000083530.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4774055
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG341800.7159532095880755No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG159010.3330711523013455No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA146400.3066575479335701No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT140920.29517883643988185No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG121320.25412359095150766No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA119360.25001806640267027No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG116510.2440482985637995No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA105950.22192873772924693No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC104620.21914284607110726No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG98460.20623976891761825No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC95990.2010659701239303No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC95280.19957876480266776No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG85550.17919776793522488No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC82080.171929313759477TruSeq Adapter, Index 6 (100% over 51bp)
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC72270.15138074446146935No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT72250.15133885135382813No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG69260.14507583176146904No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG66870.14006960539834584No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA64630.13537757734253167No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA57990.1214690656056539No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT53010.11103768180299556No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT52620.11022076620399221No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA48320.1012137480611346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC12900.033.48842629
CGTATGC13150.032.85176544
GCACACG13850.032.65279811
ACGTCTG14300.031.94027715
ACTCTAG37100.031.7187125
TACTCTA35400.031.7164864
CACACGT14400.031.40597212
CACGCCA13900.031.2410631
GTATGCC14000.031.01790845
ACACGTC14600.030.82164413
TCTAGTT37300.030.7028397
CTCTAGT39100.030.2685936
GTACTCT37200.030.0003643
CGCCAAT14950.029.1967633
CACGTCT15600.028.99012814
TCGTATG15150.028.96044243
TTGTACT39050.028.6439111
TCACGCC15250.028.62299530
TGTACTC39350.028.361212
ACGCCAA15500.028.30649832
Produced by FastQC (version 0.11.2)