FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_05.35100000083516.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_05.35100000083516.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4869879
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG193940.39824398101061653No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA96740.19864969950998784No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC96080.19729442969732922TruSeq Adapter, Index 5 (100% over 51bp)
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT88340.18140081098524213No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG83560.1715853720390178No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA71350.1465128805048339No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA70380.1445210445680478No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG67550.13870981188649656No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC63930.1312763623079752No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG63390.13016750518852727No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC57960.11901733082074523No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55900.11478724625396236No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG51060.10484860096113272No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC50400.10349333114847413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC14250.032.6846944
GCACACG14900.032.4662511
ACGTCTG15050.032.14299815
GTATGCC14900.031.40985745
TCGTATG15150.030.74302343
CACACGT16150.029.9536912
CTCGTAT15500.029.90366442
CACGTCT17550.027.82063514
CACAGTG17250.026.99956333
TACTCTA20350.026.75664
ACACGTC18400.026.29060613
AGCACAC19200.025.54672410
CACACAG18150.025.53677731
ACACAGT18350.025.38105832
TCTAGTT22350.025.0664527
TCTCGTA18900.024.88106241
ACTCTAG25100.024.5613565
TCCAGTC20200.024.17015325
GTACTCT22250.023.8650263
CCAGTCA20300.023.82941826
Produced by FastQC (version 0.11.2)