FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_04.351000000834fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_04.351000000834fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4626012
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG171030.3697136972407335No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC116170.25112342985707775TruSeq Adapter, Index 4 (100% over 51bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA87840.18988277592016622No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT81530.17624251731296847No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG71460.1544743074596434No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA64580.13960188603055937No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA63950.1382400218590008No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG61450.1328357989559906No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC60800.13143070100120796No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG53030.11463437621865226No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC52700.11392101879545491No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAG48410.10464737229388943No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46280.10004297438052474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17700.035.97471244
ACGTCTG18650.034.26323315
GTATGCC18650.034.1422245
TCGTATG18950.033.6017143
GCACACG20050.033.1041511
CACACGT20150.032.38155712
CACTGAC19900.031.77147731
CTCGTAT20100.031.67922642
ACTGACC20150.031.15396532
CACGTCT21400.030.28081714
AGCACAC22550.029.53384410
AGTCACT23050.028.21052228
ACACGTC23300.028.10035913
CAGTCAC23150.027.69989227
TCTCGTA23350.027.26991341
CTGAACT23950.027.24406819
CCAGTCA24100.026.70134726
ACTCCAG24300.026.57302723
TCCAGTC24600.026.52363225
GTCACTG24100.026.42126329
Produced by FastQC (version 0.11.2)