FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_03.351000000834d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_03.351000000834d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5608289
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG118490.2112765586794832No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC115370.2057133646286773TruSeq Adapter, Index 3 (100% over 51bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA60780.10837529948973743No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAG56320.1004227849171111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC16950.036.2393445
CGTATGC17000.036.00039744
TCGTATG18100.033.68821743
ACGTCTG18950.033.3641915
TAGGCAT18700.033.08948535
CTCGTAT18750.032.8806642
GCACACG19300.032.87513411
TTAGGCA19200.032.11058834
CACGTCT20350.030.95829814
CACTTAG20900.030.03595431
AGCACAC21700.029.75759910
CACACGT21850.029.03844812
CATCTCG21550.028.81805439
GAGCACA22500.028.1993069
TCACTTA22300.028.15028830
ACTTAGG22250.028.01129732
CAGTCAC23100.027.27278727
GGCATCT22550.027.2407437
ACACGTC24150.026.17971413
TCTCGTA24050.025.72816741
Produced by FastQC (version 0.11.2)