FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_02.351000000834b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_02.351000000834b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4108396
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG99200.24145676317472806No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC89240.2172137252592009TruSeq Adapter, Index 2 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68360.16639097107484283No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA61600.14993686100366177No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT46170.1123796245542056No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA43250.1052722278962398No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43160.10505316430061756No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG43020.10471239870742742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13300.033.83463344
ACGTCTG14600.031.59212315
GTATGCC14400.031.56254245
GCACACG14900.030.95641711
TCGTATG14650.030.71676643
CCGATGT14850.030.30270233
CACACGT15750.029.28539712
CGATGTA15800.028.62380634
CACGTCT16200.028.61080414
CTCGTAT15950.028.49533542
AGCACAC16600.027.92172610
TATCTCG16650.027.43246839
ACACGTC17150.027.41952913
CACCGAT17800.025.66045831
ACCGATG18000.025.12533832
AGTCACC19100.024.1495428
TCTCGTA19450.023.83007641
CAGTCAC19900.023.17842327
TGTATCT19750.023.12661637
TCGGAAG19750.023.1266163