FastQCFastQC Report
Tue 28 Mar 2017
HGJJWBCXY_l01n01_transplanta1_01.35100000083481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJJWBCXY_l01n01_transplanta1_01.35100000083481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207184
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG97090.4398817678997311No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC76890.3483624382924124TruSeq Adapter, Index 1 (100% over 51bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA52490.237814337182582No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT39470.1788251455247954No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA37080.16799686840788988No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG36970.16749849582091933No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC31510.14276109286765398No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG31210.14140189490318886No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA29500.13365446650573762No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC29030.13152505636140893No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG26760.12124045843028945No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25160.11399140261980877No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC23980.10864522395957925No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT23260.10538314884486295No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG22480.10184923413725364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG11500.037.7619711
CGTATGC11250.037.00024444
GTATGCC11500.036.78201745
ACGTCTG12000.035.06352215
TCGTATG12000.034.50022543
CACACGT12800.034.1025612
CTCGTAT12300.033.84168642
CACGTCT12700.033.4852314
AGCACAC12900.033.31492610
ACACGTC13250.032.60472513
GTCACAT13350.031.51706129
TCGGAAG13700.030.054263
ACTCCAG14450.029.27222323
TCTCGTA14500.029.0174341
GATCTCG14200.028.99732839
ACATCAC14550.028.45379432
GAGCACA14950.028.4450029
ACGATCT14600.028.3563537
CACGATC14600.028.201636
GAACTCC14600.028.20096221
Produced by FastQC (version 0.11.2)