FastQCFastQC Report
Wed 12 Oct 2022
HGJHJBGXM_n02_CHAR29_blank_IDT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJHJBGXM_n02_CHAR29_blank_IDT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10598740
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAGAACCGGTGTAGA919490.8675465196806413Illumina Single End PCR Primer 1 (97% over 34bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAGAACCGGTGTAG456380.4305983541439832Illumina Single End PCR Primer 1 (97% over 34bp)
GGGCGAACCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAG389770.36775126099894895Illumina Single End PCR Primer 1 (97% over 34bp)
GGCGAACCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAGA369050.3482017673798961Illumina Single End PCR Primer 1 (97% over 34bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAGAACCGGTGTA352730.3328037106297541Illumina Single End PCR Primer 1 (97% over 34bp)
AGGGACGGTCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATA317160.2992431175781272Illumina Single End PCR Primer 1 (97% over 34bp)
AGAGAAGGGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAG280750.26488997748789006Illumina Single End PCR Primer 1 (97% over 34bp)
AGGGACGGTCGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAT249790.23567895806482658Illumina Single End PCR Primer 1 (97% over 34bp)
AGGGACGGTCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAG237340.22393227874256752Illumina Single End PCR Primer 1 (97% over 34bp)
AAGGGAACGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAG225060.21234599584478908Illumina Single End PCR Primer 1 (97% over 34bp)
ACACTCTTTCCCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAT169190.1596321826934145Illumina Single End PCR Primer 1 (97% over 34bp)
AGGGACGGTCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAGA166050.15666956638241905Illumina Single End PCR Primer 1 (97% over 34bp)
GAGAGAAGGGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATA135220.12758120304866427Illumina Single End PCR Primer 1 (97% over 34bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAGAACCGGTGT132260.12478841824594245Illumina Single End PCR Primer 1 (97% over 34bp)
AGGGACGGCCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATA128790.12151444416977866Illumina Single End PCR Primer 1 (97% over 34bp)
GGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAGAACCGGTG128680.1214106582480559Illumina Single End PCR Primer 1 (97% over 34bp)
GGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAGAACCGGTGTAG124130.11711769512225038Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120650.11383428596229363No Hit
GGAGAGGGAGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATA119270.11253224439886252Illumina Single End PCR Primer 1 (97% over 34bp)
GGCGAGAGACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAG112630.10626734876032434Illumina Single End PCR Primer 1 (97% over 34bp)
AGGGACGGCCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAG111950.10562576306240175Illumina Single End PCR Primer 1 (97% over 34bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAGAACCGGTGTAG111020.10474830026965469Illumina Single End PCR Primer 1 (97% over 34bp)
AGGGAACGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATAGA107600.10152150161245582Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)