FastQCFastQC Report
Wed 12 Oct 2022
HGJHJBGXM_n02_3690_2_IDT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJHJBGXM_n02_3690_2_IDT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6469536
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAACACGGATTAGGGAGTTTATCTGTAGTGCGAGTCTTTGCACGTTAA149160.23055749284029026No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGAAGAACGTGTAGA99340.15355042463632632Illumina Single End PCR Primer 1 (97% over 36bp)
GGGCAACACGGGGAAACTTACCAGGACCAGATGTTTTTTATGACTGACAG96020.1484186810306025No Hit
GGGATGTAGTCTGCCATACGCAGGAAGACCCGCTGAACTGAAGCATCTCA95640.14783131278657388No Hit
GGAACACGGATTAGGGAGTTTATCTGTAGTGCGAGTCTTTGCACGTTAAC80040.12371830066329331No Hit
GGGGAACACGGATTAGGGAGTTTATCTGTAGTGCGAGTCTTTGCACGTTA78820.12183253945878035No Hit
GGGGTAGTCTGCCATACGCAGGAAGACCCGCTGAACTGAAGCATCTCATT71880.11110534047573117No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)