FastQCFastQC Report
Wed 12 Oct 2022
HGJHJBGXM_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJHJBGXM_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39631630
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGACGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG5807111.46527155204063Illumina PCR Primer Index 4 (96% over 29bp)
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCTCTTATACACA1488790.37565701940596435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)