FastQCFastQC Report
Wed 12 Oct 2022
HGJHJBGXM_n01_CHAR30_1_IDT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJHJBGXM_n01_CHAR30_1_IDT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8838541
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCCTTGATCGACAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCTTC420490.4757459404216149No Hit
CCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAA314310.3556129908771142No Hit
CCTCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA264570.2993367344225704No Hit
CCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAAA248190.28080426396166513No Hit
CGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAAC224330.25380885827197047No Hit
CGCCCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAAC218930.24769925262551817No Hit
CCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTCA216610.24507438501444978No Hit
ACCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC215980.24436159768903035No Hit
CCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCTGA215200.2434790990956539No Hit
CGCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT211350.23912317655142404No Hit
CTCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT209010.23647568077129472No Hit
CCCCGATCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCTGA181720.20559954408764977No Hit
GCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAA173530.19633330885719713No Hit
CGCCCCTGATCGACAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCTTC172270.19490773420635826No Hit
ACCCCTGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC167410.1894090891245512No Hit
CCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCTG164890.18655793982287347No Hit
CCGGCAGTCCGACGGTAACCGCCCGTTACCCGTGATAGCCTACCTAGGTA159800.1807990707968657No Hit
CACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTACTC159250.18017679614769No Hit
CGTACTAAATCAATCTACTATCATGATGCTATATCCAGTAGGATAAAACA156580.17715593557805523No Hit
TCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAA155290.17569641867362498No Hit
GCCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC151690.1716233482426568No Hit
CCTCGCGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA150100.1698244088023125No Hit
CTCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGA150010.1697225820415383No Hit
TTCGTTCACACTACTTGTAATCTATCGGTCTCCTCACGTTATTTAGCCTT149340.16896453837799702No Hit
ACGCCTTTACAGTTAACGTGCAAAGACTCGCACTACAGATAAACTCCCTA146070.16526483273653422No Hit
CCTCCGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTAC141580.16018480878235444No Hit
ACGGGCGTTTTAACTGCAACAATATGGGAGCAAACTCGCAGAGCTGGAAT140520.15898551582212495No Hit
CCGCCCTTGATCGACAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCTT139140.15742417215692048No Hit
CTCCCTGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT133090.15057915101598782No Hit
CCTTCAAGTTTCAGCCTTGCGGTCGTAGTTCCCCCAGAGCCCAAGAACTA132390.14978716509885512No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTATTCAACGTGCGTTGTTGACACA129820.14687944537452505No Hit
CTCGTACTAAATCAATCTACTATCATGATGCTATATCCAGTAGGATAAAA126750.14340602142367162No Hit
CACCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT124450.14080378198166416No Hit
CCGCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA120720.13658362845179992No Hit
CCCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTAC119730.13546353408328365No Hit
ACTCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGG117120.13251055802083173No Hit
TCCTTCAAGTTTCAGCCTTGCGGTCGTAGTTCCCCCAGAGCCCAAGAACT114290.1293086720987095No Hit
TGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAAC111030.1256202805417772No Hit
GCGCTGAGTCATTCATGTAGCCATACTCTAAGCGTCCTGATTTGTTCACA109100.12343666222739703No Hit
CTCGCCCTTGATCGACAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCT108930.12324432279037908No Hit
ACCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAA108120.12232788194341125No Hit
CGCTTGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAAC107830.1219997734920277No Hit
CCTCATGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA105940.11986141151576941No Hit
CCTCTCGTACTAAATCAATCTACTATCATGATGCTATATCCAGTAGGATA104890.11867343264007034No Hit
TCTCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA104480.11820955517432119No Hit
GGCCCTCTGCTAGGTTTCGGTACGGTGGAAAACCGTACCCCTATCCACAG103510.11711208897486587No Hit
CGCCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAACG102360.11581096925386215No Hit
GGTCTCCTCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGA101290.11460036220910215No Hit
GGACCTGTTATTGCTACCCTCTTCCACCTGCTAAAATCGCAGGGAGTCCC100860.11411385657429207No Hit
CCCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT100530.11374049178478665No Hit
CTCCTCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT100100.11325398614997655No Hit
GCCCCTGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC98330.11125139318808386No Hit
GGCCGAATTCCCTGAACTATCATGCCTCAATGGCCATAGGCTACTTACCT97720.11056123403172537No Hit
TCTCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTC96970.10971267769194033No Hit
CCTCCCGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA96570.10926011431072165No Hit
CCGCGTAACAATCGGTTCTGATTTTCATCAGAGCCAAGCTTTATCCCGAA96550.1092374861416607No Hit
CCCCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA94710.10715569458805475No Hit
GCGCCATTCATTTTCGGGGCCCTAGTATTCGGCAGGTGAGTTTTAACACA92980.10519835796428392No Hit
CCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCTGAA92510.10466659599135196No Hit
CGCGCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAAC92280.10440637204715121No Hit
CCCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC91630.10367095655267086No Hit
CCCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGA90960.10291291288912956No Hit
TAGCCTTGGATGGGACATACCACCCCCTTAAGACACTACTCTCAAAGTAT90780.10270925936758114No Hit
CCCCTCAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAAA90740.10266400302945927No Hit
ACCCTCTTCCACCTGCTAAAATCGCAGGGAGTCCCAATTAGAACTTTAAC90440.10232458049354526No Hit
CCTCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTC89250.10097820443441965No Hit
CCTTACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA89020.10071798049021892No Hit
CTCTCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT89010.10070666640568846No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)