FastQCFastQC Report
Wed 12 Oct 2022
HGJHJBGXM_n01_CHAR29_blank_IDT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJHJBGXM_n01_CHAR29_blank_IDT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10598740
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAAGGAACATCTCG896730.8460722689678207TruSeq Adapter, Index 22 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAAGGAACATCTC590110.5567737297074935TruSeq Adapter, Index 22 (97% over 37bp)
CCCGGTTCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTA389940.36791165742342957TruSeq Adapter, Index 22 (97% over 37bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAAGGAACATCT379180.357759507262184TruSeq Adapter, Index 22 (97% over 37bp)
CCCGGTTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAA370460.3495321141947062TruSeq Adapter, Index 22 (97% over 37bp)
CCCGACCGTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCT306270.2889683113275729TruSeq Adapter, Index 8 (97% over 36bp)
CCCCGACCGTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC243390.22964050443731993TruSeq Adapter, Index 5 (97% over 35bp)
CCGACCGTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTA230770.2177334286905802TruSeq Adapter, Index 22 (97% over 37bp)
GCCCCCTTCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTA220490.2080341625513976TruSeq Adapter, Index 22 (97% over 37bp)
GCCCGTTCCCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTA178430.16835020011812724TruSeq Adapter, Index 22 (97% over 37bp)
CGGGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC163710.15446175677486193TruSeq Adapter, Index 5 (97% over 35bp)
CGACCGTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAA160150.15110286694456132TruSeq Adapter, Index 22 (97% over 37bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAAGGAACATC140790.13283654472135367TruSeq Adapter, Index 22 (97% over 37bp)
CCCGGCCGTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCT125630.11853295769119726TruSeq Adapter, Index 8 (97% over 36bp)
AGGCTCCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCT118540.11184348328197502TruSeq Adapter, Index 8 (97% over 36bp)
CGGTCTCTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTA111340.10505022295103003TruSeq Adapter, Index 22 (97% over 37bp)
GCCCCCTTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCT109640.10344625870622358TruSeq Adapter, Index 8 (97% over 36bp)
CCGGCCGTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTA109530.1033424727845008TruSeq Adapter, Index 22 (97% over 37bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAAGGAACAT106850.10081387032798239TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)