FastQCFastQC Report
Wed 12 Oct 2022
HGJHJBGXM_n01_CHAR29_3_IDT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJHJBGXM_n01_CHAR29_3_IDT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9560378
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCCTTGATCGACAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCTTC447210.4677743913472877No Hit
CCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAA345690.361586121385577No Hit
CGCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT316810.3313781107818122No Hit
CCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAAA288270.3015257346519144No Hit
CCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCTGA282200.29517661330964107No Hit
CCTCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA271950.2844552799062966No Hit
CTCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT271120.28358711339656234No Hit
CCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCTG261950.27399544243961904No Hit
CCCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT242070.253201285555864No Hit
CCCCGATCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCTGA226890.23732325228144743No Hit
CGCCCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAAC225430.23579611601131253No Hit
GCCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC224720.23505346755117842No Hit
CGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAAC222690.23293012054544288No Hit
ACCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC220610.23075447435237395No Hit
CCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTCA219230.22931101678197244No Hit
CGTACTAAATCAATCTACTATCATGATGCTATATCCAGTAGGATAAAACA191950.200776580172876No Hit
GCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAA188420.19708425754713885No Hit
CTCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGA172530.18046357581258818No Hit
CCGGCAGTCCGACGGTAACCGCCCGTTACCCGTGATAGCCTACCTAGGTA172090.18000334296405435No Hit
CACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTACTC167590.17529641610404945No Hit
CGCCCCTGATCGACAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCTTC166210.17385295853364793No Hit
TCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAA165530.17314168958591386No Hit
ACGCCTTTACAGTTAACGTGCAAAGACTCGCACTACAGATAAACTCCCTA158580.16587210254657295No Hit
TTCGTTCACACTACTTGTAATCTATCGGTCTCCTCACGTTATTTAGCCTT157740.16499347619937205No Hit
CTCCCTGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT154740.16185552495936875No Hit
CCTCGCGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA154200.16129069373616817No Hit
CACCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT150670.15759837111043098No Hit
CCTCCGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTAC145650.15234753270215884No Hit
CCGCCCTTGATCGACAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCTT144370.1510086735064241No Hit
ACCCCTGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC138290.14464909232668416No Hit
CCTTCAAGTTTCAGCCTTGCGGTCGTAGTTCCCCCAGAGCCCAAGAACTA136760.1430487371942825No Hit
CCCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTAC135510.1417412575109478No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTATTCAACGTGCGTTGTTGACACA134250.1404233179901464No Hit
CCGCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA133210.13933549489361194No Hit
GCGCCATTCATTTTCGGGGCCCTAGTATTCGGCAGGTGAGTTTTAACACA132280.13836273000921093No Hit
CTCGTACTAAATCAATCTACTATCATGATGCTATATCCAGTAGGATAAAA131300.1373376659374765No Hit
GCCCCCAGGATACAAGCCTGGATAATCGCTACTTCAATTAGGATCTGTAA126770.1325993595650716No Hit
ACTCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGG123710.12939864930026826No Hit
TCCTTCAAGTTTCAGCCTTGCGGTCGTAGTTCCCCCAGAGCCCAAGAACT122730.12837358522853384No Hit
CCCCGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCTGAA122060.12767277611826644No Hit
CTCGCCCTTGATCGACAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCT118830.12429424861652959No Hit
CCTCTCGTACTAAATCAATCTACTATCATGATGCTATATCCAGTAGGATA118410.12385493544292915No Hit
CTCCTCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT117390.12278803202132804No Hit
CCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCTGAA117310.12270435332159461No Hit
CCTCATGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA116250.12159561055012678No Hit
CCCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC114210.11946180370692457No Hit
CCCCTCAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAAA113020.11821708304838993No Hit
ACCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAA111980.11712925995185546No Hit
TCTCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTC111190.11630293279198793No Hit
TCTCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA108900.11390763001211876No Hit
TAGCCTTGGATGGGACATACCACCCCCTTAAGACACTACTCTCAAAGTAT108740.11374027261265192No Hit
CGCCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAACG107840.11279888724065093No Hit
CCCCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA106770.11167968463171646No Hit
CCCCGTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAAA106380.11127175097051603No Hit
CCCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGA106330.11121945178318264No Hit
TGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAAC105910.11078013860958218No Hit
CTCTCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT105860.1107278394222488No Hit
CCCCCTACTCTCAGTTCAATAGAACTGATGTCTGAGATCGGTAGTACTAT105460.11030944592358169No Hit
CCTCCCGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA105120.10995381144971465No Hit
TTCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT104730.10954587778851423No Hit
CGCTTGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAAC103800.10857311290411321No Hit
GGACCTGTTATTGCTACCCTCTTCCACCTGCTAAAATCGCAGGGAGTCCC103770.10854173339171318No Hit
GGCCCTCTGCTAGGTTTCGGTACGGTGGAAAACCGTACCCCTATCCACAG103440.10819655875531281No Hit
CCTCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTC102460.10717149468357841No Hit
ACCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTCA102330.1070355167965116No Hit
GGTCTCCTCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGA101820.10650206508571104No Hit
CCCCGCTGGATATCTAGTGACCGTTCCCACTACAGTTGGTTCGCTGAGAG100860.10549792068891No Hit
GCGCTGAGTCATTCATGTGGCCATACTCTAAGCGTCCTGATTTGTTCACA100530.10515274605250964No Hit
CTTCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT98480.10300847937184072No Hit
ACTATCATGATGCTATATCCAGTAGGATAAAACAAACCTGGCTCACGACG98400.10292480067210731No Hit
CCGCGTAACAATCGGTTCTGATTTTCATCAGAGCCAAGCTTTATCCCGAA96520.10095835122837192No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)