FastQCFastQC Report
Wed 12 Oct 2022
HGJHJBGXM_n01_3690_2_IDT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGJHJBGXM_n01_3690_2_IDT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6469536
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCCTTGATCGACAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCTTC323330.4997730903730963No Hit
ACCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC211330.32665402897518464No Hit
GCCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC205110.3170397382439792No Hit
CCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTCA193750.2994805191593339No Hit
CCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAA155270.24000175592190845No Hit
ACCCCTGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC144010.22259710742779698No Hit
CGCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT137780.21296735963753813No Hit
CCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCTGA129780.20060171239483018No Hit
CCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAAA127330.19681473292675086No Hit
CGCCCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAAC126870.19610370821029513No Hit
CTCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT124450.19236309991937597No Hit
CGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAAC123480.19086376519119763No Hit
CCTCACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTA113120.1748502520118908No Hit
CCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCTG108990.1684664866228428No Hit
CGCCCCTGATCGACAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCTTC106740.16498864833583118No Hit
CCCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC100910.15597718290770776No Hit
CCGGCAGTCCGACGGTAACCGCCCGTTACCCGTGATAGCCTACCTAGGTA99240.15339585404579248No Hit
CCCCGATCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCTGA98730.15260754403406984No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTATTATCTCG97230.1502889851760621TruSeq Adapter, Index 2 (97% over 36bp)
CGTACTAAATCAATCTACTATCATGATGCTATATCCAGTAGGATAAAACA97030.14997984399499437No Hit
CCGCCCTTGATCGACAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCTT93080.14387430566890733No Hit
CCTTCAAGTTTCAGCCTTGCGGTCGTAGTTCCCCCAGAGCCCAAGAACTA90910.14052012385432278No Hit
CCCCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT88970.1375214543979661No Hit
GGCCCTCTGCTAGGTTTCGGTACGGTGGAAAACCGTACCCCTATCCACAG88630.13699591439015102No Hit
TCCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC85750.13254428138277613No Hit
ACGCCTTTACAGTTAACGTGCAAAGACTCGCACTACAGATAAACTCCCTA83420.12894278662333744No Hit
GCCCCTGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTC82220.12708793953693126No Hit
GCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAA80170.12391924243098733No Hit
GCGCCATTCATTTTCGGGGCCCTAGTATTCGGCAGGTGAGTTTTAACACA80000.12365647242707978No Hit
CTCGCCCTTGATCGACAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCT79890.12348644477749254No Hit
TCCTTCAAGTTTCAGCCTTGCGGTCGTAGTTCCCCCAGAGCCCAAGAACT78480.12130699945096526No Hit
GTCACACCCATGCTTCTCGACCGAGATCGAGTTTAGCGCAATTTGCATTC76290.11792190351827396No Hit
ACCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAACAAAGACTTCA75400.11654622526252269No Hit
GGCCGAATTCCCTGAACTATCATGCCTCAATGGCCATAGGCTACTTACCT74200.11469137817611649No Hit
ACCCTCTTCCACCTGCTAAAATCGCAGGGAGTCCCAATTAGAACTTTAAC73850.11415038110924802No Hit
ACGGGCGTTTTAACTGCAACAATATGGGAGCAAACTCGCAGAGCTGGAAT73850.11415038110924802No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTATTCAACGTGCGTTGTTGACACA73710.11393398228250064No Hit
CTCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGA73490.11359392698332615No Hit
CTCCCTGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT72580.11218733460946814No Hit
CACGTTATTTAGCCTTGGATGGGACATACCACCCCCTTAAGACACTACTC70630.10917320809405806No Hit
CCCCGCTGGATATCTAGTGCCCGTTCCCACTACAGTTGGTTCGCTGAGAG69350.10719470453522477No Hit
TCCCCTTAACTAAATGCATTACAGCTATCCTGAGGGAAACTTCGGAGGAA68980.10662279335024955No Hit
CACCCCGGTCCATCGTAAGCTTGCGGTTGGTCATACGAGGCTTTTACTCT68520.10591176863379384No Hit
GCCCGTTACCCGTGATAGCCTACCTAGGTAAAGACCCTAAGTACAAGCTG68270.1055253421574592No Hit
TTCGTTCACACTACTTGTAATCTATCGGTCTCCTCACGTTATTTAGCCTT66120.10220207446098144No Hit
CACCCATGCTTCTCGACCGAGATCGAGTTTAGCGCAATTTGCATTCAAAG66040.10207841798855435No Hit
ACTATCATGATGCTATATCCAGTAGGATAAAACAAACCTGGCTCACGACG65520.10127465091777835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)