FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46385799
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1946395941.96102992642209No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG615960.1327906413771163No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG479440.10335921991987246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT658700.069.426927
TAGTAGG651800.069.252516
TAGGTTG653650.069.234089
GGTAGTA875300.069.207434
GAGGTAG919950.069.1870352
TGAGGTA886600.069.166961
GTAGTAG875150.069.015765
TATAGTT551050.069.0139816
TGTATAG598100.068.9434914
GTTGTAT493900.068.9394612
TTGTATA600100.068.7413213
TCCCTGA490300.068.687441
AGACCCT492950.068.674897
GTATAGT598650.068.5950915
TAGTTAA548050.068.5686818
TGAGACC493650.068.422485
ATAGTTA550000.068.4147417
GAGACCC495650.068.210616
CATTGTC162450.068.129786
AGGTAGT890200.067.780383
Produced by FastQC (version 0.11.5)