FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5592503
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG585111.0462399394332018No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG422680.7557975382400332No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC419760.7505762625429079No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA410430.7338932138257235No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT319860.5719442618090683No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA315050.5633434617737353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG248350.4440766504729635No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG219600.39266854215366537No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT216290.3867499042915131No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC190170.3400445203158586No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA146100.26124259566780744No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA135330.24198467126436948No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC114480.20470261705715667No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA113200.20241383866937576No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC99930.1786856439773032No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG95680.17108618448662433No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT90740.16225293039628233No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT85330.152579265491677No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC73450.13133654108008525No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA72340.12935174107193148No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA70390.12586493024679646No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA70250.12561459511063294No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC69410.12411258429365171No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA68730.12289667077514307No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA67600.12087610860468023No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA67450.12060789238736215No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACT67270.12028603292658045No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT63960.11436739506442822No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA63730.11395613019787383No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTA56250.10058108149427904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT527850.069.502018
GGTAGTA716400.069.403354
AGTAGGT533300.069.389587
TAGGTTG528950.069.350859
TAGTAGG529600.069.338646
AGGTTGT565500.069.3308710
GTTGTGT173100.069.24995412
GTAGTAG716450.069.248915
GTTGTAT394800.069.23566412
TATAGTT438700.069.2254316
GAGGTAG759800.069.1711042
TGTATAG483250.069.0927514
GCTACAT128350.068.99592
AGACCCT376650.068.977767
CATTGTC108700.068.975746
TAGTTAA433000.068.9565918
TGAGACC377400.068.9210365
GTGTGGT171400.068.8960115
CAGCACG150500.068.887964
GGTTGTG175050.068.8202711
Produced by FastQC (version 0.11.5)