FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8965580
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG896901.0003814588682496No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG765630.8539659453153058No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA652960.7282964403864558No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT462620.5159956187998992No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG371430.41428440770145375No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC230900.25754050490877334No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC219930.24530482132778916No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC192850.21510041737400148No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA140810.15705620829884961No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT135100.15068740672661446No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA130460.14551205833866857No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT106130.11837494060618499No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG103810.11578726641221204No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA101960.11372381931788016No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC99900.11142614309392143No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC94010.10485657369629182No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA90800.10127621414342408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT819750.069.4644558
AGTAGGT829250.069.365057
GGTAGTA1100200.069.345834
TATAGTT681350.069.3410216
TAGGTTG820850.069.315939
AGGTTGT873250.069.2927510
TAGTAGG823200.069.2865756
GTTGTAT617550.069.260612
GTTGTGT259300.069.1335212
GTAGTAG1100700.069.114765
GTGTGGT256850.069.1128215
TGAGGTA1138600.069.100251
GAGGTAG1166200.069.094962
TGAGACC683500.069.032165
CAGCACG238650.069.021714
AGACCCT683300.069.0044567
GCTACAT237350.069.001582
CGTAAAT238750.068.986649
GGTTGTG260950.068.9788111
TCCCTGA686350.068.94641
Produced by FastQC (version 0.11.5)