FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7252191
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG659200.9089666833099128No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG629570.8681100649445113No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA506080.6978304901236054No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT364310.5023447396793603No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG307340.4237891693696429No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC279190.38497331358206094No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC225050.31032001225560657No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA183220.25264089155953007No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT178620.24629798084468543No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC167210.23056480448460337No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC140890.19427232404662262No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG135590.18696418778821464No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA106860.14734857369310875No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA85750.1182401290865064No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA77960.10749854768028035No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA77020.10620238766463819No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA73480.10132110420147511No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA73190.10092122504771317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT653000.069.557278
AGTAGGT660500.069.48957
AGGTTGT695550.069.4432610
TAGGTTG654100.069.402849
GGTAGTA885550.069.3720554
TAGTAGG655900.069.3480766
TATAGTT552400.069.283216
GTAGTAG884900.069.221285
GTTGTGT207200.069.2197612
GAGGTAG936750.069.167492
GCTACAT199900.069.1289752
CGTAAAT191100.069.1176769
GTTGTAT493600.069.1104512
TGAGACC512800.069.0835
CAGCACG190250.069.0477754
GTGTGGT204300.069.0393315
AGACCCT514300.068.9910357
TGTATAG605550.068.9867514
AGCTTAT146850.068.979552
GGTTGTG208550.068.939511
Produced by FastQC (version 0.11.5)