FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6694643
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG640860.9572728523388028No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG600060.8963286018388136No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA500180.7471346866442319No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT364500.5444651790991694No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC281460.42042570455213224No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG277880.41507814531708415No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC208810.3119061016397738No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA197250.2946385639981101No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT164260.2453603575276531No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC148930.22246145164126005No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC144100.21524672786883484No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG140080.20924192671662997No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA118400.17685782498036118No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA96250.14377166937803854No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA86040.12852066943674217No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA83280.12439797013821349No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA77390.11559989083809248No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT77360.11555507888919543No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA75350.11255267831309303No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC74360.11107388399949032No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC67640.10103600744655092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTGT183400.069.4248412
AGTAGGT567400.069.387727
GGTAGTA771800.069.3591164
GTAGGTT561850.069.343278
TAGGTTG561700.069.3368769
AGGTTGT597050.069.3237310
TAGTAGG562450.069.275516
GTGTGGT180800.069.24290515
TATAGTT470550.069.17969516
GTAGTAG772050.069.151295
CAGCACG182500.069.140044
GAGGTAG817350.069.138072
GCTACAT169000.069.07112
GTTGTAT418000.069.01771512
AGCTTAT135950.068.9992
AGACCCT449600.068.947487
TGAGACC449400.068.9210055
CGTAAAT183750.068.892629
GGTTGTG185250.068.8831911
TGTATAG519200.068.8721314
Produced by FastQC (version 0.11.5)