FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8369284
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG830560.9923907469264994No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG795740.9507862321316853No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA640990.7658839155177433No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT467210.5582436920529881No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG374820.44785193094176273No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC310880.37145351979930424No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC271670.32460363395482816No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA223280.2667850678743845No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC218260.26078694425950893No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT168800.20168989366354398No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG165040.19719727517909535No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC143490.17144835806742847No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA118190.14121877092472906No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA102180.12208929700557421No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA98500.11769226614845427No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA92570.11060683327271485No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA92280.11026032812364833No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT845400.069.513298
AGTAGGT855350.069.4743357
TAGGTTG845150.069.471339
AGGTTGT899550.069.4335110
TAGTAGG847550.069.428656
GGTAGTA1148000.069.4260254
GTTGTGT275650.069.3751112
TATAGTT700700.069.33958416
GTTGTAT629000.069.27946512
GAGGTAG1211750.069.26572
GTAGTAG1147300.069.249155
GGTTGTG277150.069.2277711
GCTACAT222850.069.169842
GTGTGGT272800.069.1517415
CAGCACG245750.069.133474
TGAGACC675150.069.105815
TGTATAG773450.069.0889514
TGAGGTA1188600.069.0772251
GAGACCC676050.069.055236
CTGAGAC679100.068.925074
Produced by FastQC (version 0.11.5)