FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7619006
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG699440.9180200146843303No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG549600.7213539403958994No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA497950.6535629450875875No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC401590.5270897542277825No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT350300.4597712615005159No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320140.42018604526627223No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC300290.39413277795029955No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC140600.18453850804160016No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA118340.15532209844696276No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT111020.14571454596570735No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA103590.13596261769579918No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA101640.13340322871513685No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT100780.1322744725493063No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC90860.11925440142716781No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC87700.11510687877132528No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA82350.10808496541412357No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC81760.10731058618407704No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA76580.10051179904570229No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT630900.069.461197
GTAGGTT623000.069.453458
TAGGTTG623650.069.35219
GGTAGTA842450.069.336344
TATAGTT522750.069.3087816
GTTGTAT471000.069.3064112
AGGTTGT658250.069.2796810
TAGTAGG625700.069.278496
GAGGTAG884950.069.2260742
GCTACAT221550.069.215332
GTAGTAG843300.069.075985
GTTGTGT190150.069.0587712
TGAGGTA866700.069.04161
AGCTTAT167650.069.0239942
TGTATAG577300.068.9430514
CGTAAAT186700.068.928959
AGACCCT484050.068.849647
GTGTGGT187900.068.8435415
CTGAGAC485500.068.81574
GGGCTAT56100.068.81303415
Produced by FastQC (version 0.11.5)