FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8259468
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG790720.9573497954105519No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG629030.7615865816054981No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA548160.6636747064096622No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC444290.5379160013695797No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG405470.49091539551942087No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT387730.46943701458738024No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC328040.39716843748289843No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC178300.21587346787952927No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT131620.15935651061303222No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA123420.14942851040769212No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA121470.14706758352959295No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA104180.12613403187711364No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC101320.12267133912256818No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC96110.11636342679698015No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG90790.10992233398083268No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA86370.10457089972380788No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA85980.10409871434818804No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT85600.1036386362898918No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA82880.10034544597787654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT764350.069.410888
AGTAGGT772250.069.403287
GGTAGTA1027400.069.3951954
GCTACAT247400.069.354222
TAGTAGG765850.069.3153156
AGGTTGT811550.069.2767910
TAGGTTG765650.069.274759
GTTGTAT582500.069.2459312
TATAGTT645600.069.2071816
GTAGTAG1026750.069.183875
GAGGTAG1087700.069.14262
AGACCCT588750.069.1290747
CAGCACG212650.069.116194
TGAGGTA1059900.069.110721
TGTATAG709750.069.0855614
GTGTGGT229600.069.0836415
TGAGACC589000.069.078395
CATTGTC211550.069.045796
TTGTATA709550.068.9907513
CTGAGAC590750.068.985914
Produced by FastQC (version 0.11.5)