FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4744472
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG513231.0817431317963306No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG443400.9345613168335697No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG393220.8287961231513221No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA347840.7331479667284367No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC261220.5505775985188657No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT246820.5202264867407795No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC184130.38809376470131973No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC103080.21726337514480007No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA90110.18992629738356553No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT77750.16387492644070828No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT74940.15795224421179005No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA73340.15457989845866937No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA69390.1462544198806527No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC68760.14492655874036142No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC59530.12547233917704648No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC58020.12228968787253883No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG55380.1167253173798897No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA50820.10711413198349574No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA50740.1069455146958397No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACC49910.10519611033640835No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG48380.1019713047099867No Hit
CGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCAC47880.10091744666213649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACAT131350.069.307862
GTAGGTT407150.069.2484058
TAGGTTG407000.069.230939
AGTAGGT411500.069.179767
TAGTAGG408150.069.168866
GGGCTAT37750.069.1647715
GGTAGTA547550.069.1569064
AGGTTGT430550.069.07770510
GAGGTAG576400.069.0169752
GTAGTAG547850.068.984885
AGCTACA131450.068.976791
AGACCCT328650.068.973947
TATAGTT345400.068.9554916
TGAGGTA563750.068.928721
GTTGTAT308250.068.9176312
TTGGCGA114700.068.90069617
CAGCACG126100.068.862684
CATTGTC113000.068.8546456
AAATATT126750.068.8365512
TGTATAG378250.068.8055614
Produced by FastQC (version 0.11.5)