FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6991470
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG908271.2991116317455413No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG663980.9497001345925821No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA588980.8424265569329483No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT416390.5955686000225989No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC416160.5952396277177762No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG328520.469886876436572No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC291790.4173514296707273No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC190410.2723461589622783No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA141160.20190317629911878No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT140680.20121662540209712No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA126390.1807774330720149No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA114730.16409996753186382No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC112950.16155400795540853No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC106020.15164192937965837No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG104320.14921039495270666No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT99990.1430171337358238No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC93550.13380590920078325No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA82630.11818687629354056No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA79820.11416769291722627No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA77290.11054899756417463No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA76930.11003408439140838No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT76580.1095334743623301No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG73970.10580035385977485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT661000.069.5064247
GTAGGTT653150.069.499518
TAGGTTG653650.069.414729
GGTAGTA883300.069.402034
TAGTAGG654800.069.383796
TATAGTT542600.069.3602716
AGGTTGT696800.069.305210
GTTGTAT484800.069.27592512
GAGGTAG934400.069.26642
GTAGTAG883450.069.199585
GTGTGGT210950.069.136615
CATTGTC169000.069.113276
TGTATAG596200.069.1129114
AGCTTAT160400.069.109662
TGAGGTA911750.069.097931
GTTGTGT214350.069.0834812
GGTTGTG215450.069.0399111
GCTACAT197600.069.012392
TCCCTGA549100.069.00321
TGAGACC548750.068.9003755
Produced by FastQC (version 0.11.5)