FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4701277
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG704301.4981036003621995No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG494921.0527352461894928No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA456110.9701832076688951No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC417820.8887372516020647No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT309450.6582254140736655No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG292910.6230434837172963No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC271250.5769708953546027No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC116340.24746467821402568No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA112210.23867983103314272No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC89250.18984203653603052No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA88550.18835307938672832No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT87640.18641743509263548No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT80480.17118753053691582No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC67910.14445011429873203No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG65480.13928130590901153No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA65030.1383241191701744No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA63670.13543128813724442No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA59940.12749727361310556No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG57040.12132873685171072No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG53920.11469224212910661No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA51800.11018282904836282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT448150.069.614598
AGTAGGT454350.069.5966957
TAGGTTG448300.069.544469
GGTAGTA595250.069.4666444
AGGTTGT482600.069.4468410
TAGTAGG450450.069.3801046
GAGGTAG635600.069.30312
GTTGTAT321950.069.30312
TATAGTT343550.069.2878216
GTAGTAG595150.069.2313165
GGATAGG54600.069.2287914
AGACCCT372800.069.199277
GTTGTGT164650.069.1697312
GGTTGTG165250.069.109211
CGTAAAT117100.069.1006249
TGAGGTA621500.069.090621
GATAGGC37400.069.0636715
TGTATAG376500.069.0312314
GAGACCC373850.068.999966
GTGTGGT162150.068.9418315
Produced by FastQC (version 0.11.5)