FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5147469
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG447080.8685433559677581No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG346410.6729715127959003No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC340890.6622477959556434No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG304410.5913780150982939No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA277210.5385365118274632No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC218220.42393650160884894No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT193740.37637914866510125No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA82090.1594764339522977No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC80180.15576587250938276No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC73640.14306059929646978No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT68660.133385941712325No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA63570.12349758687230558No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG63120.12262337082554553No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA59640.11586276673060102No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT54480.10583842272775222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT443450.069.435528
AGTAGGT449000.069.373647
GGTAGTA621550.069.315644
TGAGGTA634950.069.3132251
TAGGTTG443950.069.278489
GAGGTAG652850.069.273522
AGGTTGT469500.069.25043510
TATAGTT391150.069.2366816
TAGTAGG445050.069.225416
GTTGTAT341100.069.2071712
GCTACAT150300.069.141022
CAGCACG134700.069.119184
TGTATAG428350.069.0651114
GTTGTGT131100.069.0094712
AGCTTAT142350.068.9945452
CATTGTC127250.068.958776
AGACCCT346150.068.9456947
GTAGTAG623150.068.9136665
TTGTATA429600.068.8234213
TGAGACC346800.068.792215
Produced by FastQC (version 0.11.5)