FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6446534
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG657471.0198813812197376No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG490230.760455153110183No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC470890.7304545357241581No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA419720.6510785485657875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG309200.47963758509611526No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT300020.4653973747753444No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC295700.4586960993302758No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC178070.27622595335726147No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA134340.20839105168762004No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA124880.19371649943985403No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT122680.19030381287060613No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC111500.1729611602141554No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC101500.1574489485357558No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG98080.15214377214174316No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT85580.13275350754374365No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA83990.1302870658868781No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA80120.1242838399673375No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG76450.11859085828136483No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC73060.11333221852238738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT551850.069.477268
AGTAGGT558100.069.421447
TAGTAGG552650.069.35786
TAGGTTG552300.069.35049
GAGGTAG795700.069.2945252
GGTAGTA753550.069.2827764
AGGTTGT587550.069.27610
TATAGTT477900.069.2519316
GTAGTAG752150.069.198755
TGTATAG522050.069.16653414
GTTGTAT425400.069.1658212
GTTGTGT165300.069.0228212
TGAGGTA776500.068.989981
GCTACAT155300.068.9885252
AGACCCT430800.068.901617
TTGTATA523600.068.8738213
TGAGACC431150.068.842915
CAGCACG162450.068.839254
TCCCTGA433150.068.765161
GTATAGT522850.068.74715415
Produced by FastQC (version 0.11.5)