FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6395084
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG544880.8520294651329052No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC519780.812780567073083No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG388340.60724769213352No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG325450.5089065288274556No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC320030.5004312687683227No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA312210.4882031260261789No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT219380.3430447512495536No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG194090.30349874997732634No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA129680.20278076097202166No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC120630.18862926585483475No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT96080.15024040340986922No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG75400.11790306429125873No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA72830.11388435241820123No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG70030.10950598928802185No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT68740.10748881484590352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT590300.069.5602348
AGTAGGT597700.069.473067
TAGGTTG591400.069.412569
GGTAGTA785900.069.406464
TGAGGTA803300.069.4030761
AGGTTGT623100.069.3974310
GAGGTAG827950.069.3572162
TAGTAGG593000.069.3428966
GTTGTGT179850.069.3351712
GTTGTAT445950.069.3218712
TATAGTT483100.069.31744416
GCTACAT211000.069.278352
GTAGTAG785300.069.198675
AGACCCT462800.069.153077
CATTGTC183150.069.073416
TGTGGTT163050.069.0110416
CTGAGAC465350.068.9997944
TGTATAG528750.068.99232514
TTGTATA528700.068.98398613
TGAGACC463850.068.9836045
Produced by FastQC (version 0.11.5)