FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7082106
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG649080.9165070390078883No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC435440.6148453581462915No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG424050.5987625714723841No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA391290.5525051446561234No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG351810.4967590149031941No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC286460.4044842028628207No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT271920.38395358668734975No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA108540.15325949653958865No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG100990.14259882582949196No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC99860.1410032552463914No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC97880.13820747670255148No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA83920.11849582595911443No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA79200.11183114175359703No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT78730.11116749735177645No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT78220.11044737257533283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTGT186600.069.4917112
GTAGGTT619750.069.478088
AGTAGGT625950.069.4171147
TAGGTTG620250.069.3825769
AGGTTGT657450.069.3694610
GGTAGTA846350.069.322654
TAGTAGG621600.069.2955256
GTTGTAT473300.069.27344512
GAGGTAG894450.069.206732
TGAGGTA871350.069.2015841
GCTACAT206800.069.1756742
TATAGTT533250.069.1650616
GTAGTAG845900.069.1236655
CGTAAAT197900.069.095699
GGATAGG76900.069.08787514
TTGGCGA179900.069.0647817
CTGAGAC524300.069.010314
AGCTTAT196300.069.006492
TGAGACC523400.068.9952245
TGTATAG583100.068.9837414
Produced by FastQC (version 0.11.5)