FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6889464
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG589530.8556979178641474No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG416950.6051994756050689No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA380250.5519297292213153No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC356870.5179938526422375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG331860.4816920445480229No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT268680.38998679723125046No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC226340.32853063750677847No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA109140.1584158070932659No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC105670.15337913080030607No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT101460.14726835062930876No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG96280.139749623483046No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC94800.13760141572697093No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT85460.12404448299606471No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA82820.12021254483657945No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC81670.11854332935044003No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA80790.1172660166306116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT600050.069.5095147
GTAGGTT595300.069.463958
TAGTAGG596000.069.406026
GTTGTAT449750.069.39146412
TAGGTTG595800.069.388039
GGTAGTA807400.069.379784
AGGTTGT629400.069.3377310
TATAGTT501000.069.3358116
TGAGGTA827600.069.232761
GAGGTAG851850.069.15762
GTAGTAG807250.069.150865
GCTACAT190550.069.14072
GTTGTGT183050.069.13805412
TGTATAG551950.069.06682614
TTGTATA551400.069.0400113
TGAGACC449400.069.015895
AGCTTAT164300.068.938862
GGTTGTG184000.068.877211
TAGTTAA496050.068.86318
CTGAGAC451350.068.848564
Produced by FastQC (version 0.11.5)