FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9341786
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG709250.7592231292817027No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG479540.5133279653376774No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA447350.47886988633651No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC433170.4636907760464648No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG396880.4248438146624211No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT310190.3320457137425327No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC275330.29472950889690686No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA187760.2009894039533768No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC162380.17382115154425504No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT158760.16994608953791063No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC125590.134438960601324No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG105070.11247313950458725No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA102370.10958289988659556No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC96800.1036204426005905No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT96430.10322437272701386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT660800.069.431718
AGTAGGT668100.069.4229357
TAGGTTG661400.069.37939
GGTAGTA887250.069.339814
AGGTTGT701100.069.3195210
TGAGGTA912500.069.257911
TAGTAGG662350.069.2486
GTTGTAT510600.069.2372112
TATAGTT563150.069.0852216
TGTATAG616450.069.0839814
GCTACAT213250.069.034182
GAGGTAG940050.069.023322
GTAGTAG887000.069.005325
AGCTTAT181450.068.922332
TTGTATA617750.068.8876113
CTGAGAC524950.068.729064
CAGCACG185600.068.7201844
TGAGACC524550.068.6891565
GTTGTGT197550.068.6161712
GTATAGT620100.068.5584615
Produced by FastQC (version 0.11.5)