FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7880560
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG764420.9700072076096115No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC485500.6160729694336443No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG474720.6023937385160445No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA452300.573943983676287No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG361330.4585080248104195No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT315120.3998700599957363No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC306990.38955353426660033No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA179270.22748383363618827No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT164580.2088430263839118No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC161480.2049092957860863No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG124180.15757763407676612No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC116380.14767986031449543No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA110690.14045956124945436No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC109980.1395586100480169No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT98930.12553676388480006No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT88770.1126442790867654No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA88360.11212401149156913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT619800.069.387318
AGTAGGT625900.069.365297
TAGGTTG620300.069.320099
GTTGTAT473850.069.2972412
AGGTTGT654850.069.2809810
GGTAGTA840100.069.264174
TAGTAGG621600.069.230186
TGAGGTA860650.069.2168051
GTTGTGT183450.069.21670512
GAGGTAG885700.069.183582
TATAGTT526650.069.16376516
TGTATAG576900.069.1322514
GTAGTAG839850.069.055575
GCTACAT219100.069.0431062
CAGCACG183650.068.972444
TTGTATA578000.068.9639113
AGCTTAT215500.068.929642
AGACCCT495500.068.845837
GTGTGGT182250.068.7908215
CATTGTC189150.068.780316
Produced by FastQC (version 0.11.5)