FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8470357
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG804440.9497120369306747No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC669590.7905097742633516No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG500760.5911911386969876No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA470100.5549943172407019No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG422810.4991643209371222No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC356610.4210094096388145No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT322890.381199989563604No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC147210.1737943276770979No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT145640.171940804856277No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA139390.1645621312065123No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG130310.15384239412813414No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA115230.13603913034598186No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC110710.13070287356247204No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC108250.12779862761392466No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT92020.10863768788021567No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT89550.10572163605382867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT728050.069.547338
AGTAGGT736000.069.480887
TAGGTTG729250.069.38979
GGTAGTA1002350.069.379114
TATAGTT625350.069.3680116
TAGTAGG731750.069.339716
AGGTTGT770800.069.327210
TGAGGTA1030450.069.283971
GAGGTAG1058050.069.227172
GTTGTAT560150.069.2240212
GTTGTGT216400.069.2224812
GTAGTAG1000550.069.178995
TGTATAG685900.069.1368614
GCTACAT238000.069.092772
AGCTTAT231500.069.067162
TTGTATA686400.069.0300113
CATTGTC204950.068.979896
GTGTGGT214350.068.939115
CTGAGAC566750.068.904874
AGACCCT566000.068.886167
Produced by FastQC (version 0.11.5)