FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7724221
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG774411.0025735928581019No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC590200.7640899969071315No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG494960.6407895372232358No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA461570.5975618771135626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG368490.47705781592732777No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT319130.4131549317400421No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC317250.41072102934392996No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG134890.17463249692104874No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC134490.17411464534740784No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC124770.16153085210793425No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA122430.15850142040213505No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT122070.15803535398585825No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT106400.13774851858847642No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA90620.11731927400834338No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC79690.10316897975860607No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA78790.10200381371791407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT703400.069.386348
GGTAGTA972050.069.323234
AGTAGGT711200.069.2999957
TAGTAGG704450.069.278366
TAGGTTG704850.069.218329
AGCTTAT247100.069.210542
TGAGGTA997450.069.204831
GCTACAT231800.069.188392
AGGTTGT744800.069.166610
TATAGTT612250.069.16463516
GTTGTGT208750.069.1441812
GAGGTAG1026250.069.140892
GTTGTAT539650.069.1301912
GTAGTAG971000.069.077375
CAGCACG223300.069.0319444
GCTTATC247750.069.000713
TGTATAG669450.068.9954514
CGTAAAT224150.068.951759
AGACCCT517450.068.950417
CATTGTC200850.068.940756
Produced by FastQC (version 0.11.5)