FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7368870
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC619400.8405630714071491No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG616600.8367633029216148No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG436100.5918139416219855No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA384120.5212739538083858No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG347200.4711712922062677No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC340720.46237754228260236No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT268620.36453350378009114No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC131880.17896909566867103No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA131200.17804629475075556No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA121840.16534421152768333No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG121430.16478781685658725No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT109770.14896449523468322No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC108290.1469560461780436No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC106030.14388909018614793No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT96260.13063061229197964No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT87920.11931273044578071No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA82360.11176747588164807No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG75870.10296015535624864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT613550.069.392887
GTAGGTT608300.069.369948
GGTAGTA832600.069.3138054
TAGTAGG609150.069.2964866
TATAGTT530400.069.2193816
TAGGTTG609350.069.204469
GTTGTAT476500.069.2028812
AGGTTGT643100.069.1916310
TGAGGTA852200.069.177231
GAGGTAG877900.069.082252
GTAGTAG831500.069.05195
TGTATAG581050.069.0518714
TGAGACC485050.069.0002065
CAGCACG173500.068.994554
AGACCCT484800.068.9868557
CTGAGAC486700.068.953264
TTGTATA581800.068.94340513
GTTGTGT171800.068.81457512
AGCTTAT181900.068.771742
GACCCTA417300.068.764198
Produced by FastQC (version 0.11.5)