FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8305374
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG693540.8350496919223624No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC665420.8011920956238696No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG427750.5150279806785342No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG376540.45336910776083056No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA372800.448865999291543No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC316450.38101836232781333No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT261280.3145914922073347No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG176460.21246484505092725No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT166940.2010023871291046No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC145750.1754887859354678No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA124190.14952969005369293No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA95880.11544332621264257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT637350.069.415698
GGTAGTA877550.069.363824
TAGGTTG637750.069.34479
AGTAGGT644000.069.339767
TAGTAGG638850.069.235796
AGGTTGT673650.069.1976710
TGAGGTA900800.069.179371
GTTGTAT492000.069.1663112
GTTGTGT185450.069.1493412
TATAGTT554850.069.1482916
GAGGTAG927250.069.0696262
TGTATAG605750.069.0407414
GTAGTAG878850.069.0071645
GCTACAT233650.068.923412
AGCTACA233350.068.878621
TTGTATA606650.068.8682413
CAGCACG201800.068.8399054
CTGAGAC528900.068.7843254
AGACCCT528200.068.771537
TGAGACC528400.068.744265
Produced by FastQC (version 0.11.5)