FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8812918
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC853100.9680108222951808No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG720970.8180831819835384No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG485200.5505554459941645No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC424570.4817587092039209No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG391210.4439051855469437No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA387970.4402287641845754No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT267720.3037813355349499No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT200030.2269736312081878No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG198370.22509003260894972No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC189130.214605423538492No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA160020.18157436617474484No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA111180.12615571823089694No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG94050.10671834232430166No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT89100.1011015874651279No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT661450.069.298728
AGTAGGT669350.069.234157
GGTAGTA886050.069.210284
TAGGTTG661850.069.209249
AGGTTGT699800.069.1523410
TGAGGTA910750.069.142251
GTTGTAT504900.069.1110712
CAGCACG191500.069.108694
TAGTAGG663750.069.1078346
GCTACAT249450.069.106672
TATAGTT552700.069.0302716
GAGGTAG938300.068.978842
TGTATAG602700.068.9008214
GTAGTAG887600.068.8366555
TTGTATA603550.068.751213
AGACCCT547800.068.738937
GTGTGGT197500.068.6515515
CTGAGAC549550.068.647664
TGAGACC548700.068.6132665
AGCTTAT214300.068.6000442
Produced by FastQC (version 0.11.5)