FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8809539
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1078811.2245930235395972No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG714920.8115294114709067No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC604460.6861426006514075No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG496260.5633211908137304No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA450500.511377496597722No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG422730.47985484825028873No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT318840.36192586240891833No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC231760.263078465286322No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA216270.2454952523622405No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT199930.22694717623703126No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA181390.20590180712066775No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG178960.20314343349862007No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT139980.158895942228078No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC134950.15318622234375714No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG131080.14879325694568127No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC131010.14871379762323544No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT112200.12736194254886662No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA100600.11419439768641697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT656700.069.326127
GTAGGTT649200.069.322578
TAGGTTG649150.069.295569
GGTAGTA852450.069.1954964
GTTGTAT482850.069.186212
TATAGTT524900.069.1457416
TAGTAGG651700.069.145046
AGGTTGT688000.069.1066210
TGAGGTA876150.069.083681
AGCTTAT180300.069.072552
GAGGTAG902550.069.0077062
GCTACAT232250.068.96452
GTTGTGT207550.068.9356612
CTGAGAC518800.068.884424
TGTATAG574450.068.865214
GTAGTAG853700.068.856385
AGACCCT517550.068.83457
TTGTATA574000.068.7480713
CATTGTC197000.068.742896
TGAGACC518550.068.728645
Produced by FastQC (version 0.11.5)