FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8436856
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC861551.0211742383655713No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG759440.9001457414942248No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG495910.5877900488049103No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC463950.5499086389527094No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA446910.5297115418350153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG428190.5075231816212106No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT320730.3801534600092736No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC197500.23409194135825007No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT189920.22510755191270304No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA182370.21615872073672943No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG145230.17213758300485396No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC126490.14992551727800024No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT108510.12861426104700613No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG101210.11996174878414424No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA95160.11279083108684089No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA92500.10963799785133231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT714100.069.496578
AGTAGGT721900.069.429667
TAGTAGG715750.069.369866
TAGGTTG715200.069.365219
GGTAGTA963850.069.318834
AGGTTGT757250.069.2847210
GTTGTAT539000.069.2753212
GAGGTAG1016350.069.226852
GCTACAT233950.069.212222
TGAGGTA990250.069.212141
TATAGTT594150.069.1397416
GTAGTAG962400.069.1036155
TGTATAG648400.069.0581114
AGCTTAT195600.069.0209662
TTGTATA648300.068.9325713
GTTGTGT223600.068.9160712
CGTAAAT202650.068.908459
TGAGACC557250.068.86245
AGACCCT558200.068.8181467
CTGAGAC559900.068.779884
Produced by FastQC (version 0.11.5)