FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8648131
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG869261.005142035891917No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC831880.9619188238476036No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG561960.6498051428684417No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA522050.6036564432245534No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC420980.4867872607387654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG415750.4807397112740313No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT370530.42845095662866345No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG201690.23321802132738276No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC179900.20802182575633973No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT169130.19556826787198298No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA166270.192261194933333No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC140060.1619540684571036No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT115710.13379769571020605No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA106890.12359896028401975No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA99960.11558566816344479No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG98830.11427902745691526No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA91710.10604603468656985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT731850.069.394848
AGTAGGT740750.069.328097
AGCTTAT222400.069.281522
GCTACAT257550.069.2582
TAGGTTG733750.069.2385949
GGTAGTA1005500.069.224924
TAGTAGG734200.069.208926
AGGTTGT775800.069.20792410
TGAGGTA1029400.069.202831
GAGGTAG1057500.069.197872
GTTGTAT555000.069.1906212
CAGCACG218800.069.188824
AGCTACA257750.069.153161
TATAGTT616950.069.1249816
GTAGTAG1004250.069.060535
GTTGTGT224550.069.03145612
TGAGACC618250.069.002995
AGACCCT618100.068.945817
TCCCTGA621050.068.934471
CTGAGAC620650.068.9218754
Produced by FastQC (version 0.11.5)