FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11728888
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1218841.0391777975883136No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1091360.9304888920415986No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG711810.6068861771039163No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG669380.5707105396521819No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC619490.5281745379442621No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA617630.5265887098589397No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT433950.3699839234546361No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC274450.2339949021595227No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT272480.2323152885422727No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG264820.2257844051371281No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA241060.20552673024075258No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC173550.14796799150951054No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT145640.12417204427222768No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG141120.12031831150574547No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA123450.10525294469518338No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA120690.10289978043954381No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT967400.069.357758
GGTAGTA1282400.069.289264
AGTAGGT981050.069.288767
TAGGTTG967850.069.2818159
TAGTAGG970800.069.258336
GTTGTGT324850.069.211912
AGGTTGT1031300.069.1799310
GTTGTAT710500.069.1609912
TATAGTT772050.069.1192516
GCTACAT312800.069.109182
TGAGGTA1326200.069.094471
GAGGTAG1362900.069.084952
GTAGTAG1281700.069.078895
GTGTGGT320200.069.0365315
AGACCCT774050.068.9090047
TGTATAG848000.068.8548614
TGAGACC775000.068.825885
CTGAGAC777600.068.807024
AGCTACA314100.068.7700651
GAGACCC777300.068.72136
Produced by FastQC (version 0.11.5)